WebTransformed fibroblast cells were maintained as subconfluent monolayers, trypsinized, and seeded in 96-well plates (for analytical assays; Fig. 1) or in 384-well white plates (for screens; Fig. 3). For many experiments, the cell concentration was optimized to 1,000 cells per well in 100 μL of medium (for 96-well plate) or 50 cells per well in 30 μL of growth … WebJan 31, 2024 · Proteolysis with the use of trypsin – or trypsinization – is a process where you expose cells to trypsin in oder to digest intercellular and cell-to-substrate linking proteins. The cells detach from the growth vessels and from each other. The cells are said to be trypsinized. The number of cells adhering to the in vitro culture vessel will ...

Cytotoxicity assay - cellular density? ResearchGate

WebTrypsinization definition: Collins Dictionary Definition Meaning, pronunciation, translations and examples WebThe volume of trypsinized red cells is slightly larger than that of untreated cells, the increase observed after trypsinization for one hour varying from 8 to 12 per cent. This volume increase can be easily detected either by measurements made in Hamburger hematocrit tubes or by measurement of the Hb concentrations in equal volumes of untreated and … gates 9605

What is the trypsin mechanism in cell culture? ResearchGate

WebMeaning of trypsinized for the defined word. Grammatically, this word "trypsinized" is a verb, more specifically, a verb form. Webtrypsinized: trypsinized (English) Verb trypsinized Past of trypsinize. trypsinise: trypsinise (English) Verb trypsinise (third-person singular simple present trypsinises, present … Webtrypsin: [noun] a proteolytic enzyme that is secreted in the pancreatic juice in the form of trypsinogen, is activated in the duodenum, and is most active in a slightly alkaline medium. gates 9580