2024 Top/fop flash reporter assay

Top/fop flash reporter assay

Author: jdml

August undefined, 2024

WebI have been trying to measure the Wnt activity using TOP/FOP-FLASH assay but the readings that I got are rather similar in both TOP and FOP, I couldn't see a high TOP/FOP ratio. Web12. júl 2024 · The Dual-Luciferase Reporter Assay (Promega) was applied according to the manufacturer’s instructions. For the TOP/FOP-Flash assay, TOP/FOP-Flash (Genechem) was co-transfected into cells along with LUCAT1 silence or overexpression vector, miR-5582-5p mimic or inhibitor, and/or the miRNA control.

Luciferase Assay System Protocol - Promega

Web• Established the use of the TOP/FOP FLASH Luciferase Assay in Dr. Wei’s lab. • Used TOP/FOP FLASH Luciferase Assay to monitor levels of canonical Wnt-activated transcription as a proxy for ... Web26. dec 2024 · topflash is a tcf reporter plasmid containing two sets of three copies of wild-type tcf binding sites driven by the thymidine kinase minimal prompter and upstream of a … bitesize addition and subtraction

The Luciferase Reporter Assay: How it Works - Bitesize Bio

WebTOPFlash/FOPFlash assays. TOPFlash is a luciferase reporter that contains a minimal fos promoter coupled to Tcf-binding sites upstream of a modified firefly luciferase gene. … Web29. jún 2024 · TOP/FOP-Flash luciferase reporter assay and western blot were used to analysis the activation of Wnt/β-catenin signaling pathway. Si-CASC11-transfected HeLa cells were subcutaneously inoculated into male athymic (nude) mice to investigate the effect of CASC11 on the tumor formation. WebThe Luciferase Reporter 1000 Assay System (Cat.# E4550) was designed to meet the needs of users who perform a large number of assays, particularly in 96-well plates. The system contains sufficient reagents to perform 1,000 luciferase assays (100µl per assay). bitesize adjectives ks1

Autophagy influences Wnt and Notch signaling pathways.

WebTOP or FOP plasmid + Opti-MEM and Lipofectamine + Opti-MEM are incubated at room temp for 5 min respectively. Both solutions are then mixed and incubated for another 15 … WebFOP-flash reporter was adopted as the control of TOP flash reporter. g The protein expressions of β-catenin, cyclin D1 and c-myc were measured by western blot in sh-NC or … bitesize adding and subtracting fractionsWebI have been trying to measure the Wnt activity using TOP/FOP-FLASH assay but the readings that I got are rather similar in both TOP and FOP, I couldn't see a high TOP/FOP ratio. dash notary

"Web9. apr 2024 · Download Citation Mechanism of VPS26 gene promoting implant osseointegration through Wnt/β-catenin pathway in hyperlipidemia rats Objective: To investigate the mechanism of VPS26 effect on ... " - Top/fop flash reporter assay

Top/fop flash reporter assay

The Luciferase Reporter Assay: How it Works - Bitesize Bio

Web11. apr 2024 · 1.5 × 10 4 SW620 or Difi cells were plated in 24-well plates, then co-transfected with the firefly luciferase reporter plasmid TOP-FLASH or FOP-FLASH and the Renilla luciferase vector phRG-TK at 1 µg/ml (kindly provided by Dr P. Blache, IRCM, Montpellier, France) and incubated with oxaliplatin (5 µg/ml). 72 h post-transfection, cells … Web86. Millipore luciferase top flash. Luciferase Top Flash, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, …

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WebFOP-flash reporter was adopted as the control of TOP flash reporter. g The protein expressions of β-catenin, cyclin D1 and c-myc were measured by western blot in sh-NC or sh-circAGFG1#1 group. h The nuclear translocation of β-catenin in sh-NC or sh-circAGFG1#1 group was assessed by IF assay (scale bar = 20 μm). i Luciferase reporter assay ... WebTOPflash (TCF Reporter Plasmid) Transfection grade T-cell factor reporter plasmid containing 2 sets (with the second set in the reverse orientation) of 3 copies of the TCF binding site (wild type) upstream of the Thymidine Kinase minimal promoter and …

WebDescription; Catalogue Number: 17-285: Brand Family: Upstate: Trade Name: Upstate; Description: TCF Reporter Plasmid Kit: Overview: FOPflash: Transfection grade T cell factor (TCF) reporter plasmid containing two full and one incomplete copy of the TCF binding site (mutated) followed by three copies in the reverse orientation, upstream of the Thymidine … Web目的. 研究VPS26在高脂环境中对大鼠骨髓间充质干细胞（bone marrow mesenchymal stem cells，BMSC）成骨、成脂分化作用的机制，探讨VPS26对高脂大鼠种植体骨结合和裸鼠异位成骨的影响。

Web17. júl 2024 · TOP/FOP-flash reporter assays. Cells were seeded in a 96-well plate, and the TOP/FOP-Flash (Genechem) was co-transfected into cells along with LINC01278 siRNA or overexpression plasmid. Luciferase activity was measured using the Promega Dual-Luciferase Reporter Assay System at 24 h after transfection. The luciferase activity was … Web31. máj 2024 · In vitro proliferation, migration and invasion assays, and an in vivo nude mice model were utilized to assess the important role of desmosomal genes on SOX30-induced tumor suppression. A WB assay and TOP/FOP flash reporter assay was used to investigate the downstream pathway regulated by the SOX30-desmosomal gene axis.

Web6. apr 2024 · (O) Wnt activity based on a transient transfection TOP/FOP-flash reporter assay measured at day 3 of differentiation in Tet1 KO and WT ESCs, treated with 2 μg/ml DOX from day 1 to over-express Tcf7l1. TOP-Flash contains a minimal fos-promoter coupled to Tcf-binding sites upstream of a luciferase reporter; FOP-Flash contains mutated Tcf …

Web开馆时间：周一至周日7:00-22:30 周五 7:00-12:00; 我的图书馆 bitesize adjectives ks2Web背景技术微小RNA（miRNA）在肿瘤的发生和发展中起着至关重要的作用。以前，我们表明miR-504被下调并抑制胶质母细胞瘤（GBM）中的肿瘤增殖。然而，miR-504在GBM间充质（ME）过渡中的调控及其相关机制仍不清楚。方法转录组和临床数据来自癌症基因组图谱（TCGA）数据库。 bitesize adding fractions quizWeb6. apr 2024 · The TOP/FOP-FLASH assay showed that the canonical Wnt activities were reduced in HONE1 and NPC43 cells, when THY1 was over expressed (Figure 2A). ... Luciferase reporter HONE1 cells were injected into nude mice via tail vein, followed by treatment with 10 mg/kg of KX2-391 or solvent control at a frequency of twice per week … dash northridgeWebPočet riadkov: 10 · TOPflash (TCF Reporter Plasmid) 21-170 Sigma-Aldrich TOPflash (TCF … dash no-mess nonstick waffle makerWebReporter Assays State-of-the-art bioluminescent assays. Detect firefly, Renilla, NanoLuc® and click-beetle luciferase activities. Transfection Reagents Proven, well-cited … dash north hollywoodWebF , The expression of β-catenin by nuclear/cytoplasmic protein fractionation and TOP/FOP-flash reporter assays when silencing MBD2a or overexpressing MBD2c. Data were from 3 … bitesize advanced informationWebFOP-flash reporter was adopted as the control of TOP flash reporter. g The protein expressions of β-catenin, cyclin D1 and c-myc were measured by western blot in sh-NC or sh-circAGFG1#1 group. h The nuclear translocation of β-catenin in sh-NC or sh-circAGFG1#1 group was assessed by IF assay (scale bar = 20 μm). i Luciferase reporter assay was … dash northridge schedule