Web이중 가닥 cDNA 합성 원리 1. PrimeScript RTase와 Oligo(dT) 18 Primer 또는 Random Primer를 이용해 1st strand cDNA를 합성한다. 2. coli RNase H로 mRNA-cDNA hybrid의 RNA에 nick을 형성하고, E. coli DNA Polymerase I과 E. coli DNA Ligase의 시스템에 의해 RNA를 DNA로 교체하면서 이중 가닥 cDNA를 합성한다. 3. WebMixed lineage leukemia 5 (MLL5) transactivates the expression of E6 and E7 oncogenes in cervical cancer cells. In this study, we utilized CRISPR/Cas9 system with the aim to target HPV-E6 and MLL5 to enhance apoptosis efficiency in HPV-18 positive
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Web이 제품은 PrimeScript RTase를 이용하여 total RNA 혹은 Poly A + RNA로 부터 1 st strand cDNA를 합성하기 위한 모든 시약이 포함된 all-in one kit이다. 다카라에서 개발한 M … WebHowever, for RT-PCR reactions where the reverse primer for PCR is also used as a reverse transcription primer, we recommend performing the reverse transcription reaction at 50℃ to reduce the possibility of non-specific amplification products. *5 For synthesis of longer cDNAs, inactivation at 70℃ for 15 min is recommended to minimize cDNA ... mercedes benz sydney service centre
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