Stat3-y705f
WebNational Center for Biotechnology Information WebNov 5, 2013 · Background STAT3 is a transcription factor of central importance in chronic inflammation and cancer. In response to cytokine stimulation STAT3 is phosphorylated on a single tyrosine residue at position 705, dimerizes and accumulates in the nucleus to induce target gene expression. The substitution of tyrosine 705 to phenylalanine leads to a …
Stat3-y705f
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WebNov 5, 2024 · Signal transducer and activator of transcription 3 (Stat3), a transcription factor that controls normal cell differentiation and survival is also involved in neoplastic … WebMar 15, 1996 · Mutation of Stat3 tyrosine 705 to phenylalanine (Y705F) inhibits IL-6-induced tyrosine phosphorylation of this Stat3 mutant in transfected HepG2 cells. In …
WebSTAT3 (Y705F) Alt name STAT3 Alt name signal transducer and activator of transcription 3 (acute-phase response factor) Species H. sapiens (human) Insert Size (bp) 2351 Mutation Tyrosine 705 to Phenylalanine Entrez Gene STAT3 ( a.k.a. ADMIO, ADMIO1, APRF, HIES) Promoter TA Luciferase Tag / Fusion Protein FLAG (C terminal on insert) WebJun 11, 2024 · Wild-type STAT3 plasmids (pGV492-Flag-STAT3WT), constitutively active STAT3 plasmid (pGV492-Flag-STAT3-CA), dominant-negative STAT3 vector (pGV492-Flag-STAT3-Y705F) or the respective empty vectors plasmids were constructed by GeneChem.
WebNov 11, 2024 · STAT3 is a latent transcription factor, and is activated upon phosphorylation of Tyr705. We found that SD-36 also effectively degrades CRISPR-mutated homozygous Y705F mutant STAT3 protein in DLD-1/STAT3 Y705F/Y705F cells (Figure 2 H), demonstrating that phosphorylation of Tyr705 is not required for SD-36-induced STAT3 … WebFeb 22, 2024 · First, using Western blotting, we found that expressing an unphosphorylatable mutant STAT3 (STAT3 Y705F), representing uSTAT3, causes a notable increase in the …
WebMay 6, 2016 · We also demonstrated that the mutant STAT3 Y705F could form complexes with NFκB, and these unphosphorylated STAT3–NFκB complexes translocated into the nucleus and upregulated the activity of...
naruto various x chubby readerWebFeb 1, 2003 · Interferon γ (IFNγ), which also activates STAT3 in M1 cells, similarly induced the LSSIG expression (data not shown). To investigate whether the induction of LSSIG depends on STAT3 activation, we analyzed its induction in M1/Y705F cells that express dominant negative form of STAT3 (Y705F). naruto various x black readerWebSTAT3 Dominant Negative (Y705F) Lentivirus. This STAT3-Dominant Negative (STAT3-DN) Lentivirus can be used to transduce cells with a STAT3-DN gene that contains a Tyr to … naruto vector freeWebOct 7, 2024 · b, Wild-type APT2 could remove the DHHC7-introduced palmitoylation on wild-type STAT3 better than that on the Y705F mutant. DHHC7-knockout HEK293T cells were … naruto uzumaki powers and abilitiesWebSilke Metz is an academic researcher from RWTH Aachen University. The author has contributed to research in topic(s): Fusion protein & Glycoprotein 130. The author has an hindex of 4, co-authored 5 publication(s) receiving 274 citation(s). melodic bandpass filterWebaffected by Y705F STAT3, including several well-known oncopro-teins such as MRAS and MET.9 The exact mechanism underlying the transcriptional ability of unphosphorylated STAT3 is yet to be fully understood. However, one hypothesis suggests that the poten-tial for protein-protein interactions is the primary mechanism, melodica with headphonesWebendogenous Stat3 that is activated through the Jak/Stat path-way. Mutation of tyrosine 705 to phenylalanine (Y705F) func-tions as a dominant negative Stat3 for cytokine signaling. The fact that the Y705F forms of Stat3 with a MLS did not alter the induction of endogenous Stat3 is consistent with a MLS pre-/). (), melodica world