WebbPierce 蛋白酶抑制剂片剂通过抑制细胞裂解物样品中通常存在的丝氨酸蛋白酶、半胱氨酸蛋白酶、天冬氨酸蛋白酶及氨基肽酶来防止蛋白发生蛋白水解降解。 该片剂含有 AEBSF、抑肽酶、苯丁抑制素、E-64、亮肽素和抑胃肽 A。 在 4°C 下储存时可保持稳定长达 12 个月，每粒片剂足以处理 50 mL 溶液，它适用于从组织和培养细胞中提取蛋白之前的试剂制备。 … WebbA: 蛋白酶抑制剂Cocktail是低毒，全面的蛋白保护试剂，最大限度地保护蛋白，使其免于被蛋白酶降解。 目前生物学研究日渐深入，更多地将目标锁定为细胞中较为微量的蛋白，如信号通路蛋白、受体蛋白，采用coIP,Pull-down等方法获得样品。 对于这些微量的珍贵样品，Cocktail能取得最佳的保护效果，比单一的抑制剂（如PMSF）更为可靠。 Q: 蛋白酶 …
Results - randr19.nist.gov
Webbför 2 dagar sedan · To prepare soluble and insoluble samples, cells were lysed in Triton buffer (1% Triton X-100, 50 mM Tris-HCl, pH 8.0, and 150 mM NaCl) supplemented with 2 mM PMSF and protease inhibitor cocktail (Roche). After incubation on ice for 20 min, the soluble extract was collected after centrifugation at 20,000 × g for 20 min at 4°C. Webb产品说明 本产品可以方便地提供额外的 Protease Inhibitor Cocktail，以便与我们的 SimpleChIP ® Enzymatic Chromatin IP Kits (#9002, #9003)、SimpleChIP ® Plus Enzymatic Chromatin IP Kits (#9004, #9005)、SimpleChIP ® Plus Sonication Chromatin IP Kit (#56383) 和 CUT&RUN Assay Kit (#86652) 一起使用。 这些 Simplechip ® 和 CUT&RUN … add series to dataframe in specified column
APExBIO - Inhibitor Cocktails - Kits
WebbProteoGuard EDTA-Free Protease Inhibitor Cocktail은 lysis buffer에 곧바로 첨가할 수 있는 100 x stock의 protease inhibitor로 endogenous proteases로부터 protein 분해를 방지한다. Lysis buffer 1ml 당 100X protease inhibitor cocktail 10 ul를 첨가하여 사용한다. EDTA-free 제품으로 추가적인 gel filteration 과정없이 IMAC resin에 곧바로 lysate를 적용할 수 있다. … WebbGoldBio Protease Inhibitor Cocktails ProBlock™ Gold Protease Inhibitor Cocktail [100X] ProBlock™ Gold Bacterial 2D Protease Inhibitor Cocktail [100X] ProBlock™ Gold 2D … WebbDocument: Bacterial pellets were thawed on ice, resuspended in buffer A [20 mM HEPES pH 7.4, 10 mM imidazole, 0.05% Tween-20, 5 mM b-mercaptoethanol and EDTA-free protease inhibitor cocktail (Roche)] containing 500 mM NaCl, and lysed by sonication. The supernatant was cleared by ultracentrifugation at 20 000 g for 30 min and subsequently … add semicolon to excel column