Myotube fusion index analysis automated
WebMyoblast fusion is essential for formations of myofibers, the basic cellular and functional units of skeletal muscles. Recent genetic studies in mice identified two long-sought membrane proteins, Myomaker and Myomixer, which cooperatively drive myoblast fusion. WebSep 1, 2024 · Reproducibility and sensitivity of the fully automated MyoScreen platform are highlighted in assays that quantitatively measure myogenesis, hypertrophy and atrophy, AChR clusterization, and intracellular calcium release dynamics, as well as integrating contractility data.
Myotube fusion index analysis automated
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WebDec 16, 2024 · ViaFuse can detect the borders of myotubes and identify nuclear clumps which have been limitations of previous muscle-centric imaging software. The ViaFuse macros require little computer power or space to run and user inputs to the ViaFuse macros are minimal, thereby automating the analysis process in a quick, easy, and accurate fashion. WebDec 1, 2024 · A novel software tool, JADE, is presented in this work. Its aim is to lead the path to a full standardization and automatization of V&V processes for neutron-gamma Monte Carlo transport for...
WebApr 13, 2024 · Here, we describe a method that enables scientists to record myoblast differentiation and myotube formation by time-lapse microscopy and to perform quantitative analyses from the automatic tracking of myoblast nuclei.
WebFeb 15, 2011 · Methods: We have developed a simple method to quantify myotube formation using micrographs of Jenner–Giemsa-stained C2C12 cultures. Because myotubes are darkly stained by Jenner–Giemsa dyes, the extent of myotube formation correlates with an increase in pixels attributed to the darkest tones. WebJun 10, 2024 · The Myotube Analyzer can be used for the analysis of fixed differentiated myoblast cultures with nuclear and MyHC staining. The app can calculate the fusion index, an already existing parameter, but also provides multiple new parameters to comprehensively describe myogenic potential in its output.
WebFusion index is a method for determining muscle cell myotubule formation. Myotubules are multinucleated cells which occur from cells fusing together and are the precursor to muscle fibers. The fusion index is calculated by staining the fixed cell and determining the number of nuclei in a myotubule compared to total number of nuclei in the sample.
http://libna.mntl.illinois.edu/pdf/publications/129_bajaj.pdf track shoes wichita ksWebJan 21, 2024 · It is often desirable to characterise the morphology of myogenic cultures. To achieve this, the surface area of myotubes is often quantified, along with the nuclear fusion index (NFI). Existing methods of such quantification are time-consuming and subject to error-prone human input. track shoes womenWebMyoCount: A software tool for the automated quantification of myotube surface area and nuclear fusion index [version 1; referees: 2 approved] David Murphy, Thomas Nicholson, Simon Jones, Mary O'Leary Inflammation and Ageing Immunology and Immunotherapy Research output: Contribution to journal › Article › peer-review 2 Citations (Scopus) track shoes women near meWebFeb 14, 2024 · Fluorescent tagging of both the cell cytoplasm and nucleus enables monitoring of cell fusion and the formation of new myotube fibers, similar to immunostaining results. This labeling approach allowed monitoring the effects of Myf5 overexpression, TNFα, and Wnt agonist on myoblast differentiation. track shoes with spikes ratedWebfusion tube. [ ′fyü·zhən ‚tüb] (analytical chemistry) Device used for the analysis of the elements in a compound by fusing them with another compound; for example, analysis of nitrogen in organic compounds by fusing the compound with sodium and analyzing for sodium cyanide. McGraw-Hill Dictionary of Scientific & Technical Terms, 6E ... track shoes without spikesWebDec 16, 2024 · MyoCount can count MYH-positive cells with less than three nuclei in them to generate fusion index automatically; however, this number is not fully accurate because of the aforementioned issues with resolving nuclear clumps, counting myoblasts as myotubes, and defining myotube borders. the roman colony of philippiWebMar 1, 2024 · The average myotube width measured by the users were: 11.5, 9.4, 11.0, 11.5 and 12.2 µm. The macro (yellow line) calculated the average myotube width to be 11.7 µm. For optimal macro performance, the staining should label the whole cytoplasm of the myotube, as with a desmin antibody or with cytoplasmic GFP. trackshop24