2024 Klf4 phase separation

Klf4 phase separation

Author: kdba

August undefined, 2024

WebApr 13, 2024 · Many proteins undergo a process of phase separation that leads to the formation of biomolecular condensates. It has been observed that some of these proteins form dense droplets of sizeable dimensions already below their critical concentration for phase separation. To understand this phenomenon, which is not readily compatible with … WebApr 10, 2024 · Liquid‒liquid phase separation (LLPS) facilitates the formation of membraneless biomolecular condensates, which are crucial for many cellular processes in plants, including embryogenesis, the floral transition, photosynthesis, pathogen defense, and stress responses. The main component required for LLPS is a protein harboring key ...

N6-methyladenosine RNA methylation modulates liquid‒liquid phase …

WebFor this ratio, we observe four categories of ligand-modulated phase behaviors of scaffolds: 1) Ligand binding abolishes scaffold phase separation (phase diagrams with red bounding boxes in Fig. 2 C ); 2) ligand binding destabilizes scaffold phase separation (phase diagrams with blue bounding boxes in Fig. 2 C ); 3) ligand binding does not impact … WebApr 4, 2024 · KLF4 is a therapeutically tractable brake on fibroblast activation that promotes resolution of pulmonary fibrosis. Epithelial-mesenchymal reprogramming by KLF4-regulated Rictor expression contributes to metastasis of non-small cell lung cancer cells. the brick whitehorse yt

Klf4 Is a Transcriptional Regulator of Genes Critical for EMT, Including …

WebSep 5, 2006 · We show that overexpression of KLF4 induces arrest and apoptosis in the G 1 phase of the cell cycle. KLF4-mediated death, but not cell-cycle arrest, can be rescued by Bcl-X L overexpression. Transformed pro/pre-B cells expressing KLF4 display increased expression of p21 CIP and decreased expression of c-Myc and cyclin D2. Tetracycline … WebAug 26, 2024 · Admittedly, phase separation is a young biological research direction, and many problems persist to overcome how to precisely regulate phase separation, such as … Web788 Likes, 28 Comments - Virginie Lascension (@virginie_lascension) on Instagram: "C’est une résurrection pour nous rendre meilleur. Une entrée dans une octave ... the brick wigan contact

Effect of the molecular weight and its distribution of …

Surface condensation of a pioneer transcription factor on DNA

WebJan 14, 2024 · Phase separation describes the large-scale, three-dimensional concentration of proteins and nucleic acids ( Box 1) and is a means by which transcriptional machinery and multiple regulatory elements are brought together. WebApr 17, 2024 · The relationship between the molecular weight of PVDF and its distribution, phase separation, crystallization behavior and spinning process has been systematically studied. The effects of three factors on the microstructure and properties of the PVDF membrane have been analyzed. The flow behaviors of the PVDF/diluent and PVDF melt … the brick wine fridgeWebHere we investigate the role of DNA in nucleation of condensates, using the pioneer transcription factor KLF-4. We show that KLF-4 forms liquid-like condensates on the DNA surface at physiological concentrations, below the one required for Klf4 phase separation. the brick williams lake bc

"WebAug 10, 2001 · Krüppel-like factor 4 (KLF4) is an epithelial cell-enriched, zinc finger-containing transcription factor, the expression of which is associated with growth arrest. … " - Klf4 phase separation

Klf4 phase separation

Complex assembly process involved in DNA virus replication

WebDec 21, 2024 · Phase separation Germ granules are the earliest known and most studied phase-separated membrane-less organelles in germ cells during early embryonic … WebKLF4 belongs to the Kruppel-like factor (KLF) family, a group of conserved zinc finger TFs that regulates essential biological processes such as proliferation and development. …

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WebSep 22, 2024 · KLF4 is thought to induce long-range pluripotency contacts through condensation at promoters of pluripotency factors (Sharma et al. 2024 ). However, KLF4 condensation depends on KLF4-DNA... WebSep 23, 2024 · The study (“ Liquid condensation of reprogramming factor KLF4 with DNA provides a mechanism for chromatin organization ”), published in Nature Communications, shows that the binding of KLF4 can...

WebTo evaluate the role of KLF4 in cell-cycle progression, we silenced KLF4 by siRNAs in MCF-7 cells. FACS analysis showed a significant increase in the proportion of cells at S phase (Fig. 5E). Moreover, cell-proliferation analysis showed that silencing KLF4 promotes MCF-7 cell growth (Fig. 5F). Together the results indicate that KLF4 has an ... WebKLF4 is thought to induce long-range pluripotency contacts through condensation at promoters of pluripotency factors (Sharma et al. 2024 ). However, KLF4 condensation …

WebSep 22, 2024 · The study, published in the journal Nature Communications, reveals that the binding of KLF4 can cause DNA to condense into a separate liquid phase in a process … WebDec 17, 2024 · Phase separation promotes cell fate transitions, whereas disruption of phase attenuates the transitions. The major challenge is how to effectively predict mutation residues. For complete details on the use and execution of this protocol, please refer to Wang et al. (2024). Graphical abstract Download : Download high-res image (225KB)

WebDec 7, 2024 · KLF4 plays a critical role in determining cell fate responding to various stresses or oncogenic signaling. Here, we demonstrated that KLF4 is tightly regulated by …

WebFeb 25, 2013 · We have identified the zinc-finger transcription factor Kruppel-like factor 4 (Klf4) among the transcription factors that are significantly downregulated in their … the brick windsor ontario canadaWebAug 28, 2013 · KLF4, as a member of KLF family, expresses in a wide range of tissues in mammals, and plays a critical role in regulating a diverse array of cellular processes … the brick windsor ontario flyer the brick winnipeg reclinersWebAug 17, 2024 · Liquid-liquid phase separation (LLPS) is an important organizing principle for biomolecular condensation and chromosome compartmentalization. However, while many proteins have been reported to undergo LLPS, quantitative and global analysis of chromatin LLPS property remains absent. Results the brick wombleWebKlf4 forms phase separated condensates in vitro. a, SDS gel showing recombinantly expressed and purified MBP-Klf4-GFP. b, Test of the affinity of Klf4-GFP to short dsDNA … the brick wing back chairsWebKLF4 belongs to the Kruppel-like factor (KLF) family, a group of conserved zinc finger TFs that regulates essential biological processes such as proliferation and development. Reflecting pioneer factor activity, KLF4 uses two out of its three zinc fingers to target a GC-rich hexameric motif enriched within nucleosomal targets22 ( Fig. 7.2 C). the brick windsorWebApr 11, 2024 · Phase separation of biomolecules occur to form membraneless compartments in response to extra- or intra-cellular stimuli. Recently, phase separation has been identified in different immune signaling pathways, of which cyclic GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING) signaling is an important innate immune … the brick wood fired eats