Hygromycin b resistance cassette
Web1 dec. 2003 · Therefore, the aim of this work was to design a more efficient gene knockout method for use in this yeast. To simplify the use of multiple selection markers, we have developed disruption cassettes containing the homologous ylURA3 and ylLEU2 genes, and the heterologous marker hph conferring resistance to hygromycin-B (Hyg) into the … WebCochliobolus heterostrophus was transformed with a plasmid (pH1S) containing a bacterial gene (hygB), which confers resistance to the antibiotic hygromycin B when under control of an 838-bp fragment of promoter 1 from C. heterostrophus. The plasmid integrated at either homologous (52% single copy, 33% tandemly repeated copies) or ectopic (4% …
Hygromycin b resistance cassette
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Web1 okt. 1991 · Hygromycin resistance gene cassettes were designed to facilitate vector construction for plant transformation. Unique EcoRI, Pstl, and Sacll sites in the coding … Web29 mrt. 2024 · A new expression cassette (EC0) consisting of the fused 5′ and 3′ intergenic regions (IGRs) of the Eremothecium cymbalariae translational elongation factor 1α (EcTEF1) gene was evaluated through expression of the bacterial hygromycin B phosphotransferase (hph) resistance gene in the common baker’s yeast …
WebName Vector Type Resistance Marker Bacterial Resistance Source Sequence Available; 7749 Bicistronic_ires_hygro: Retroviral: Hygromycin: Addgene: bRA66: Yeast Expression, CRISPR, Synthetic Biology Web24 dec. 2024 · Hygromycin B- and nourseothricin-resistant transformants with red ascospores were isolated, analyzed by PCR for correct integration of the deletion cassette, and crossed to wildtype to dispose of the NHEJ-deficiency and to recycle the nat1 marker.
WebPromega hygromycin b resistance gene cassette Hygromycin B Resistance Gene Cassette, supplied by Promega, used in various techniques. Bioz Stars score: 86/100, … Web1 okt. 1991 · Hygromycin resistance gene cassettes were designed to facilitate vector construction for plant transformation. Unique Eco RI, Pst l, and Sac ll sites in the coding sequence of a hygromycin B phosphotransferase gene ( …
WebWe have utilized the promoter cassettes to express the hygromycin-resistance gene either from the CaMV 35S or the CaMV 19S promoters, with both chimeric resistance …
Web11 jan. 2024 · Using a method based on homologous recombination described previously 11, the target genes were deleted with a hygromycin B resistance cassette or the promoters of the target genes were replaced ... cute ghost shower curtainsWebA binary vector containing a hygromycin B resistance cassette was introduced into A. tumefaciens, and the resultant strain was co-cultivated with fungal small conidia. … cheap baby girl clothes websitesWeb21 apr. 2014 · The hygromycin B (Hyg R) resistance marker used in this study confers resistance by the activity of a specific a minoglycoside ph osphotransferase encoded … cute ghost symbol copy pasteWebHygromycin B resistance as dominant selectable marker in yeast. Saccharomyces cerevisiae is normally sensitive to the drug hygromycin B; a hygromycin B concentration … cute ghost pumpkin carving templatescheap baby girl clothes setWeb26 jan. 2024 · Primary transformants were crossed with the color spore mutant fus1-1 (S23442) and single-spore isolates (ΔSmlon2) with hygromycin resistance were selected . The absence of the Smlon2 gene and the integration of the hph -cassette at the desired locus was verified using primer pairs lon2-ko-v5f/tC1_0 (1400 bp), h3_0/lon2-ko-v3r … cheap baby girl clothing onlineWebhygromycin B 70-O-kinase or simply hygromycin phosphotrans-ferase [14]. The specific modification of hygromycin B is a phosphorylation at the 70-OH of the destomic acid ring [15]. Resistance to apramycin (AprR) is conferred by the 777 bp aac(3)IV aminoglycoside 3-N-acetyltransferase gene [13]. Specifically, the cheap baby girl clothes online